Cell Culture Technology Laboratory
Head of laboratory: Bettina Weiss
Scientific officer: Mareike Schultze
Objectives
- Training in basic eucaryotic cell culture technology
- characterisation of cellular markers by immunocytochemistry
- proliferation measurement and cytotoxicity testing in microplates using chromogenic substrates
- scale-up of adherent cells in roller bottles for production of cell mass
- scale-up hybridoma cells in bioreactors and production of monoclonal antibodies
- differentiation studies in transfilter systems
- Giemsafärbung
During a 6 week laboratory practical, students learn and practise the basic techniques of eukarotic cell culture with human and animal cell-lines. Working independently, they plan, carry out and evaluate experiments. At the same time, they learn the principles involved in working with a QM system in the laboratory based on SOPs and lists. These basic techniques are applied in further complex experiments, e.g. in cytotoxicity testing of unknown probes, double- and triple-fluorescence staining of fixed cells and differentiation studies to form epithelial barriers.
giemsa stain
Experiments
E1: Sterile working methods
E2: Adherent cell cultivation
E3: Suspension cell cultivation
E4: Comparsion of coating methods
E5: Immunohistochemistry
E6: Proliferation and cytotoxicity tests
E7: Cryopreservation
- Fluoreszenz, Kerne
Fluorescence, cell nucleus
- Fluoreszenz, Mitochondrien
Fluorescence, mitochondria
- Fluoreszenz, Cytoskelett
Fluorescence, cytoskeleton
- Fluoreszenz, überlagert
Fluorescence, overlay
Laboratory apparatus and equipment
- laminar flow work benches class II
- CO2 incubators
- upright microscopes ZEISS Axiostar plus brightfield and phase contrast
- inverted microscopes Leitz und ZEISS Axiovert
- ZEISS Axio Imager microscope with brightfield, phase contrast and epifluorescence, imaging system AxioVision
- liquid nitrogen storage system
- -80°C freezers New Brunswick and Fryka
- analytical balances
- microplate reader Tecan CM Genios (absorption, fluorescence and luminescence
- bioreactor Minifors (2L)
- wave bioreactor (2L)
- steam autoclave
- voltohmmeter for electrical resistance measurement
- multi channel pipette
- Arbeiten an der Sterilbank
Working at the laminar flow work bench
- Produktion von monoklonalen Antikörpern im 2 l Wave-System
Production of monoclonal antibodies in 2 L Wave-System
Kontaktadresse
Hochschule Esslingen
Angewandte Naturwissenschaften
Kanalstr. 33
73728 Esslingen
Telefon +49(0)711.397-35 01
Telefax +49(0)711.397-35 02
E-Mail
